Discourse Journal of Agriculture and Food Sciences
Volume 4 Issue 3 Page 31 - 38. October 2016

Copyright 2016 Discourse Journals


Transcriptional and translational characterization of cysteine proteinase inhibitor genes in Rice (Oryza sativa L. indica)

Yalu Guo1#, Jinping Lan2#, Shijuan Dou1#*, Wei Fan1, Lijuan Liu1, Liyun Li1 and Guozhen Liu1

1College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China
2Life Science Research Center, HeBei North University, Zhangjiakou 075000, China

Abstract

Cysteine protease inhibitor (cystatin) genes exist in animals, plants and microorganisms. There are 12 cysteine protease inhibitors in rice (Oryzacystatin, OC) which associated with biotic/abiotic stresses responses and normal growth and development. Oryzacystatin belongs to pathogenesis-related gene family 6 (PR6), namely protease inhibitor class in rice genome. In this study, the domain architecture and subcellular localization of oryzacystatin was predicted, and transcription analysis was also carried out. Furthermore, oryzacystatin expression profiling was surveyed in different tissues during rice normal growth using western blot (WB) technology, most of the proteins were detected in late stage of rice growth but merely in the seedling stage.

Keywords: Rice; Oryzacystatin; Transcription; MPSS; Western blot

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