Discourse Journal of Agriculture and Food Sciences
Volume 5 Issue 1 Page 1 - 11. April 2017

Copyright 2017 Discourse Journals


Application of retrotransposon based molecular markers

Tadesse Abate

Department of Animal and Range Science, college of  Agriculture and natural resources, Madda walabu University, P.O. Box 247, Bale Robe, Ethiopia

Abstract

Molecular markers are indispensable tools for determining the genetic variation and biodiversity with high levels of accuracy and reproducibility. The dynamism and dispersion of the various groups of transposable elements have led to their widespread exploitation as molecular markers. With regard to their special utility as genetic markers for molecular ecologists and conservation biologists, retrotransposons offer certain advantages over microsatellites and sequence data which are currently in wide use. In recent years, several retrotransposons based molecular marker have been developed. The SSAP, IRAP, and REMAP methods are multiplex and generate anonymous marker bands; RBIP scores individual loci, much as microsatellite-based marker systems do. The methods are variously suited to marker detection on agarose and polyacrylamide slab gels, slab and capillary sequencing devices, and arrays on solid supports. This short review narrates the application of these molecular markers for genetic diversity, gene mapping and phylogenetic studies.

Keywords: Molecular markers, Transposable elements, Retrotransposon

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