Discourse Journal of Agriculture and Food Sciences
Volume 6 Issue 4 Page 25 - 34. October 2018

Copyright 2018 Discourse Journals


Eziashi E. I., Chidi N.I. and Airede C.E

Cultural and Media Studies of Fusarium oxysporium f. sp. elaeidis of the oil palm from African Countries

Nigerian Institute for Oil Palm Research (NIFOR), Plant Pathology Division, P.M.B. 1030

Benin City, Edo State. Nigeria.

 

Abstract

Fusarium oxysporum f. sp. elaeidis species from African countries produced visible growths in culture media. The objective of this study was to determine the best supporting medium for the growth and sporulation of F. oxysporium f. sp. elaeidis. The four species were grown on different media and at six days produced various morphological structures. Microscopic view of Fusarium species shows macro conidia were absent on sporodochia, macro conidia small, macro conidia elongated and slender on sporodochia and macro conidia large on aerial mycelia. In some cases, it was straight on the right (ventral) and slightly curved on the left (dorsal), the apical cell was blunt. The overall mean of spore counts on best supporting media for F. oxysporium f. sp. elaeidis species, Malachite Green had the highest with 11.94. These were followed by Home Green (11.13); V8 Juice medium and Potato Dextrose Agar (6.56); Malachite Green Captan Medium (2.31) and Park’s Medium (2.13) respectively. Mean counts of Fusarium oxysporum f. sp. elaeidis species in broth sporulation in different media, F. oxysporium isolate 1 had the highest counts. This was followed by F. oxysporium isolate 4. The least isolate was F. oxysporium CRT. Six days after incubation in different selective media, the highest mean linear growth in centimeter (cm) was recorded by Malachite Green (4.5). These were followed by Malachite Green Captan and V8 Juice medium with 4.3 each, Home and Mitters (4.26), Komada medium (4.23), PDA (3.6) and Park medium (3.25). Malachite Green was found to be most suitable for spores production.

Keywords: Oil Palm, Culture, Media, Spore, Fusarium oxysporium

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