Journal of Microbiology Research and Reviews
Volume 4 Issue 2 Page 12 - 18. July 2017
Copyright 2017 Discourse Journals


Isolation and Characterization of Cellulolytic and Proteolytic Microorganisms Associated with Municipal Solid Wastes in Jos Metropolis, Nigeria

1Egbere, O.J, 1Okechalu, J. N, 1Zi, S.K, 2Danladi, M.M, 2Entonu, E.E, 2Yakubu, E


1Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria.

2Department of Microbiology, Plateau State University, Bokkos, Plateu State Nigeria


Abstract

The research was conducted to isolate, identify and screen for effective cellulolytic and proteolytic bacteria and fungi associated with segregates of biodegradable municipal solid wastes from three locations in Jos Metropolis, Nigeria. Thirty samples of the segregated wastes were collected and cultured in Nutrient agar fortified with meat extract for proteolytic bacteria, Carboxymethyl cellulose agar (CMCA) for cellulolytic organisms and Sabouraud dextrose agar (SDA) for fungi growths. Microbial loads were determined for each sample respectively. The growths were purified by repeated sub-culturing and identified using standard microbiological techniques. The various isolates of bacteria and fungi were tested for their proteolytic and cellulolytic potentials by measuring the zones of clearing of the individual organisms cultured on Cesien agar and CMCA respectively. The results of the study showed that cumulative mean counts for cellulolytic and proteolytic bacteria were 2.9 x 109 Cfu/g and 4.4 x 109 Cfu/g respectively. The percentage frequencies (%) of occurrence and zones of clearing (in millimeters) for cellulolysis and proteolysis (all in brackets) of the bacteria and fungi involved were: Bacillus sp. (6.67:39:21), Coagulase negative Staphylococcus (33.33:20:19), Bacillus subtilis (6.67:17:0), Aeromonas schubertii (13.33:16:0) and Klebsiella aerogenes (16.67:8:22), Staphylococcus aureus (6.67:0.00:20.00), Rhizopus sp. (13.33:43:36), Mucor sp. (30.00:29:52), Fusarium sp. (6.67:29:16), Aspergillus niger (13.33:28:29), Aspergillus fumigatus (3.33:26:32) and Cylindrocarpon sp. (3.33:20:0). While 5 (55.6%) of the isolated bacteria and 6 (100%) fungi had cellulolytic activity, 4 (44.4%) bacteria and 5 (86.3%) fungi demonstrated proteolytic activity when grown on their respective selective media. The results of this study imply that a high percentage of cellulolytic and proteolytic microorganisms which hold promise for use as industrial and bioremediation agents can be sourced locally from municipal solid wastes.

Keywords: Municipal Solid Wastes, Cellulolytic, Proteolytic Microorganisms.

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